Characterisation of Mytilus edulis hemocyte subpopulations by single cell time-lapse motility imaging.
Identifieur interne : 000955 ( Main/Exploration ); précédent : 000954; suivant : 000956Characterisation of Mytilus edulis hemocyte subpopulations by single cell time-lapse motility imaging.
Auteurs : Frank Le Foll [France] ; Damien Rioult ; Sofiane Boussa ; Jennifer Pasquier ; Zeina Dagher ; François LeboulengerSource :
- Fish & shellfish immunology [ 1095-9947 ] ; 2010.
English descriptors
- KwdEn :
- MESH :
- cytology : Hemocytes, Mytilus edulis.
- instrumentation : Video Recording.
- metabolism : Actin Cytoskeleton.
- methods : Video Recording.
- Animals, Cell Movement, Cell Shape.
Abstract
In bivalve molluscs, defence against pathogens mainly relies on fast tissue infiltration by immunocompetent hemocytes that migrate from circulating hemolymph to sites of infection, in order to deliver, in situ, an effective immune response. In the present work, we have investigated dynamics of hemocyte subpopulations motility by combining flow cytometry coupled to Coulter-type cell volume determination, Hoffman modulation contrast microscopy, time-lapse imaging and off-line analysis of cell shape changes. Our results revealed fast modifications of hemocyte aspect in vitro, with bidirectional transitions from spread outlines to condensed cell body morphologies, in the minute range. Amoeboid or non-amoeboid types of locomotion were observed, depending on the cell shapes and on the cell subtypes, with velocities reaching up to 30 mum min(-1). Correlations between motion profiles, Hemacolor staining and flow cytometry analysis on living cells help to propose a functional mussel hemocyte classification including the motile properties of these cells. In particular, basophils were shown to be involved in dynamic hemocyte-hemocyte interactions and in the constitution of aggregation cores. Physiological implications, in terms of immune response in organisms devoid of endothelium-closed vascular system, and potential applications of hemocyte motility studies for the development and the interpretation of experiments involving hemocytes in the field of marine ecotoxicology are discussed.
DOI: 10.1016/j.fsi.2009.11.011
PubMed: 19944763
Affiliations:
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last="Rioult">Damien Rioult</name></author><author><name sortKey="Boussa, Sofiane" sort="Boussa, Sofiane" uniqKey="Boussa S" first="Sofiane" last="Boussa">Sofiane Boussa</name></author><author><name sortKey="Pasquier, Jennifer" sort="Pasquier, Jennifer" uniqKey="Pasquier J" first="Jennifer" last="Pasquier">Jennifer Pasquier</name></author><author><name sortKey="Dagher, Zeina" sort="Dagher, Zeina" uniqKey="Dagher Z" first="Zeina" last="Dagher">Zeina Dagher</name></author><author><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:19944763</idno><idno type="pmid">19944763</idno><idno type="doi">10.1016/j.fsi.2009.11.011</idno><idno type="wicri:Area/PubMed/Corpus">000229</idno><idno type="wicri:Area/PubMed/Curation">000229</idno><idno 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540, 76058 Le Havre cedex</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Rioult, Damien" sort="Rioult, Damien" uniqKey="Rioult D" first="Damien" last="Rioult">Damien Rioult</name></author><author><name sortKey="Boussa, Sofiane" sort="Boussa, Sofiane" uniqKey="Boussa S" first="Sofiane" last="Boussa">Sofiane Boussa</name></author><author><name sortKey="Pasquier, Jennifer" sort="Pasquier, Jennifer" uniqKey="Pasquier J" first="Jennifer" last="Pasquier">Jennifer Pasquier</name></author><author><name sortKey="Dagher, Zeina" sort="Dagher, Zeina" uniqKey="Dagher Z" first="Zeina" last="Dagher">Zeina Dagher</name></author><author><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name></author></analytic><series><title level="j">Fish & shellfish immunology</title><idno type="eISSN">1095-9947</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Actin Cytoskeleton (metabolism)</term><term>Animals</term><term>Cell Movement</term><term>Cell Shape</term><term>Hemocytes (cytology)</term><term>Mytilus edulis (cytology)</term><term>Video Recording (instrumentation)</term><term>Video Recording (methods)</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Hemocytes</term><term>Mytilus edulis</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Video Recording</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Actin Cytoskeleton</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Video Recording</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Movement</term><term>Cell Shape</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In bivalve molluscs, defence against pathogens mainly relies on fast tissue infiltration by immunocompetent hemocytes that migrate from circulating hemolymph to sites of infection, in order to deliver, in situ, an effective immune response. In the present work, we have investigated dynamics of hemocyte subpopulations motility by combining flow cytometry coupled to Coulter-type cell volume determination, Hoffman modulation contrast microscopy, time-lapse imaging and off-line analysis of cell shape changes. Our results revealed fast modifications of hemocyte aspect in vitro, with bidirectional transitions from spread outlines to condensed cell body morphologies, in the minute range. Amoeboid or non-amoeboid types of locomotion were observed, depending on the cell shapes and on the cell subtypes, with velocities reaching up to 30 mum min(-1). Correlations between motion profiles, Hemacolor staining and flow cytometry analysis on living cells help to propose a functional mussel hemocyte classification including the motile properties of these cells. In particular, basophils were shown to be involved in dynamic hemocyte-hemocyte interactions and in the constitution of aggregation cores. Physiological implications, in terms of immune response in organisms devoid of endothelium-closed vascular system, and potential applications of hemocyte motility studies for the development and the interpretation of experiments involving hemocytes in the field of marine ecotoxicology are discussed.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Le Havre</li></settlement></list><tree><noCountry><name sortKey="Boussa, Sofiane" sort="Boussa, Sofiane" uniqKey="Boussa S" first="Sofiane" last="Boussa">Sofiane Boussa</name><name sortKey="Dagher, Zeina" sort="Dagher, Zeina" uniqKey="Dagher Z" first="Zeina" last="Dagher">Zeina Dagher</name><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name><name sortKey="Pasquier, Jennifer" sort="Pasquier, Jennifer" uniqKey="Pasquier J" first="Jennifer" last="Pasquier">Jennifer Pasquier</name><name sortKey="Rioult, Damien" sort="Rioult, Damien" uniqKey="Rioult D" first="Damien" last="Rioult">Damien Rioult</name></noCountry><country name="France"><region name="Région Normandie"><name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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